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Spectrophotometry

Spectrophotometry

Ultraviolet-visible spectroscopy (UV-VIS or UV/VIS) is a technique based on the measurement of light absorption or reflectance in the ultraviolet and visible spectral regions. Since absorption in the visible range directly influences the perceived color of substances, UV/VIS spectroscopy is an essential analytical tool.

It is widely used in materials science and analytical chemistry to characterize optical and electronic properties, as well as to perform quantitative analyses of various analytes such as transition metal ions, highly conjugated organic molecules, and biological macromolecules.

Instrument characteristics

  • Model: PerkinElmer Lambda 950
  • Detector: Peltier-controlled PbS
  • Spectral Range: 175-3300 nm
  • Max Resolution: down to 0.05 nm
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