Università Cattolica del Sacro Cuore

Spectrophotometer

SPECTROPHOTOMETRY

Ultraviolet–visible spectroscopy or ultraviolet–visible spectrophotometry (UV-VIS or UV/VIS) refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. The absorption or reflectance in the visible range directly affects the perceived color of the chemicals or materials involved. UV/VIS spectroscopy is routinely used in materials science and analytical chemistry for the characterization of the optical/electronic properties and the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules.

Instrument characteristics

  • Model: PerkinElmer Lambda 950 
  • Detector: Peltier-controlled PbS
  • Spectral Range: 175-3300 nm
  • Max Resolution: down to 0.05 nm

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